We must also realise that the church would have been strictly compartmentalised. The south chapel was probably almost entirely cut off from the rest of the church, and the nave would be divided from the chancel by the rood screen, through which what took place at the altar could be only partially glimpsed. The altars at the ends of the aisles were probably also surrounded by screens. The congregation would be confined to the nave itself and would see and hear little of the offices in the chancel, except at the elevation of the Host. In the fifteenth century Titchfield was a prosperous town due to being a thriving sea port. There was also a tannery providing employment. The church was again enlarged inkeeping with the needs of the growing population. The north aisle with tall columns and fine traceried windows was added and it is possible that a spire was added to the tower.

In medieval times the interior of the church would have been magnificent, with coloured murals on the walls, stained glass windows, at least three altars with brightly coloured hangings, each with a reredos and niches with brightly painted statues. The church would have been divided into strict compartments and the nave would have been cut off from the chancel by a rood screen. The altars would have been hidden from the congregation who would be restricted to the nave so that they would be able to see and hear very little of what the priest was doing apart from elevating the Host. Rich ornamentation was greatly loved by the people in the pre-reformation period.

The Survey of 1546 gives the first picture of a street system in Titchfield. Many streets developed around the area of the church, including the High Street, West Street, South Street and East, now Church, Street.

On 28 December 1537 the abbey was finally formally surrendered to the Crown. At this time it had in its possession 16 manors, various other areas of land and the churches of Titchfield, Lomer and Corhampton. The site of the abbey and the estates were granted to Thomas Wriothesley. The tenants came in such large numbers to renew their leases with the new landlord, that the parish church had to be used as the manor court. Wriothesley was a powerful man, but he was in some disgrace when he died in 1550. One of the reasons for this was that, in the developing divisions of those years, some of the family remained Catholics or Catholic sympathisers.

Thomas' son Henry was involved in several plots against the queen and spent several years in the Tower. On his death he left money for the erection of a monument in the parish church. The massive Southampton Monument dominates the South Chapel and was built in 1594 by a famous sculptor of the period, Gerard Johnson, a Flemish refugee.

Henry's son, another Henry, spent much time improving the interests of Titchfield, building the canal, ironworks and the Stony Bridge. He made a great map, built the market hall, and revived industries. The population of the village rose steadily from the late 1500s to the 1640s and again it was a time of prosperity for the village.